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微流体平台,用于用动态压缩刺激软骨细胞
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Bioengineering
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JoVE Journal
Bioengineering
A Microfluidic Platform for Stimulating Chondrocytes with Dynamic Compression
Please note that all translations are automatically generated.
Click here for the English version.
微流体平台,用于用动态压缩刺激软骨细胞
DOI:
10.3791/59676-v
•
07:23 min
•
September 13, 2019
•
Donghee Lee
,
Alek Erickson
,
Andrew T. Dudley
,
Sangjin Ryu
4
1
Department of Genetics, Cell Biology and Anatomy
,
University of Nebraska Medical Center
,
2
Department of Physiology and Pharmacology
,
Karolinska Institutet
,
3
Department of Mechanical and Materials Engineering
,
University of Nebraska-Lincoln
,
4
Nebraska Center for Materials and Nanoscience
,
University of Nebraska-Lincoln
Chapters
00:04
Title
00:46
Microchannel Layer Preparation
01:48
Thin PDMS Membrane Preparation
02:31
Tubing Block Preparation
03:23
Alginate-Chondrocyte Construct
04:23
Device Assembly and Actuation
05:24
Results: Representative Alginate Gel and Chondrocyte Deformation Under Static Compression
06:40
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文提供了用于软骨细胞压缩的气动微流体装置的制造和特征的详细方法。
Tags
Microfluidic
Chondrocytes
Dynamic Compression
Mechanobiology
Hydrogel
PDMS
Transparency Film
Plasma Cleaner
Spin Coating
Agarose Gel
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