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生物层干涉测量(BLI)在转录中研究蛋白质-蛋白质相互作用的应用
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription
DOI:

07:18 min

July 26, 2019

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Chapters

  • 00:04Title
  • 00:59Biosensor Hydration and Assay Set-Up
  • 02:51Loading of Ligand onto Biosensor and Washing Away Additional Ligand
  • 03:43Association of Analyte to Ligand and Dissociation of Analyte from Ligand
  • 05:41Results: A 28 Amino Acid Middle Region of GrgA Binds σ28 In Vitro
  • 06:45Conclusion

Summary

Automatic Translation

转录因子(TFs)与RNA聚合酶的相互作用通常使用下拉测定进行研究。我们应用生物层干涉测量(BLI)技术来描述GrgA与衣原RNA聚合酶的相互作用。与下拉检测相比,BLI可检测实时关联和解结,具有更高的灵敏度,并且具有高度定量性。

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