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Brug af invertebrate Galleria mellonella som en infektions model til undersøgelse af Mycobacterium tuberkulose komplekset
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Immunology and Infection
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JoVE Journal Immunology and Infection
Use of the Invertebrate Galleria mellonella as an Infection Model to Study the Mycobacterium tuberculosis Complex

Brug af invertebrate Galleria mellonella som en infektions model til undersøgelse af Mycobacterium tuberkulose komplekset

DOI:
10.3791/59703-v

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09:23 min

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June 30, 2019

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, , , , , ,
1Section of Pediatric Infectious Diseases and Allergy, Department of Medicine,Imperial College London, 2Department of Pharmacy,National University of Singapore, 3MRC Centre for Molecular Bacteriology and Infection, Department of Medicine,Imperial College London

Chapters

  • 00:04Title
  • 00:59Preparation of M. bovis BCG lux for Infection
  • 03:09Infecting G. mellonella with BCG lux
  • 05:23Measuring the In Vivo Burden of BCG lux in G. mellonella
  • 07:10Results: BCG lux Dose Dependent Virulence
  • 08:31Conclusion

Summary

Automatic Translation

Automatic Translation

Galleria mellonella blev for nylig etableret som en reproducerbar, billig, og etisk acceptabel infektion model for Mycobacterium tuberkulose kompleks. Her beskriver og demonstrerer vi de skridt, der er taget for at etablere en vellykket infektion af G. mellonella med bioluminescent Mycobacterium bovis BCG Lux.

Tags

Keywords: Galleria MellonellaTuberculosisInfection ModelMycobacteriumInnate ImmunityAlternative To Mammalian ModelsBioluminescenceBCGCell CultureSample Preparation

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