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A Double Humanized BLT-mice Model Featuring a Stable Human-Like Gut Microbiome and Human Immune System
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Biochemistry
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JoVE Journal Biochemistry
A Double Humanized BLT-mice Model Featuring a Stable Human-Like Gut Microbiome and Human Immune System

A Double Humanized BLT-mice Model Featuring a Stable Human-Like Gut Microbiome and Human Immune System

DOI:
10.3791/59773-v

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00:07 min

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August 30, 2019

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, , ,
1Nebraska Center for Virology, 2School of Biological Sciences,University of Nebraska-Lincoln, 3Department of Food Science and Technology,University of Nebraska-Lincoln

Chapters

  • 00:04Title
  • 00:41Bone Marrow, Liver, Thymus-Humanized (hu-BLT) Mouse Generation
  • 03:43Human Immune Cell Level Evaluation and Fresh Fecal Sample Collection
  • 05:20Fecal Transplant
  • 06:05Results: Representative Double Humanized BLT-Mouse Gut Microbiome Analyses
  • 07:02Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a novel method for generating double humanized BLT-mice that feature a functional human immune system and a stable engrafted human-like gut microbiome. This protocol can be followed without the need for germ-free mice or gnotobiotic facilities.

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Keywords: Double Humanized MiceHuman Gut MicrobiomeHuman Immune SystemBiomedical ResearchHematopoietic Stem CellsTransplantationSurgery Procedure

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