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在定义良好的剪切应力下单细胞电电流的电生理记录

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Immunology and Infection

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JoVE Journal Immunology and Infection

Electrophysiological Recordings of Single-cell Ion Currents Under Well-defined Shear Stress

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在定义良好的剪切应力下单细胞电电流的电生理记录

DOI:

10.3791/59776-v

•

07:17 min

•

August 02, 2019

•

Ibra S. Fancher, Irena Levitan

¹Department of Medicine, Division of Pulmonary, Critical Care, Sleep and Allergy,University of Illinois at Chicago

Chapters

00:04Title
00:29Assembly of the Modified Parallel Plate Flow Chamber
01:43Cell Preparation and Seeding into the MPP Flow Chamber
03:43Controlling Shear Stress to the MPP Flow Chamber for Electrophysiological Recordings
05:46Results: Example of Leak Subtraction for Accurate Analysis of Mechano-activated Kir Current
06:43Conclusion

Summary

Automatic Translation

该协议的目的是描述一个经过修改的平行板流室,用于研究通过剪切应力实时激活美能敏感子孔通道。

Tags

Keywords: Electrophysiological Recordings Single-cell Ion Currents Shear Stress Parallel Plate Flow Chamber Mechanosensitive Ion Channels Silicone Elastomer Cover Glass Cell Culture Electrophysiological BAF Solution Cell Adhesion

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