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In Vitro Generation of Heart Field-specific Cardiac Progenitor Cells
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Developmental Biology
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JoVE Journal Developmental Biology
In Vitro Generation of Heart Field-specific Cardiac Progenitor Cells

In Vitro Generation of Heart Field-specific Cardiac Progenitor Cells

DOI:
10.3791/59826-v

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09:29 min

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July 03, 2019

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, ,
1Division of Cardiology, Department Medicine,Johns Hopkins School of Medicine

Chapters

  • 00:04Title
  • 00:44Mouse Embryonic Stem Cell (mESC) Maintenance
  • 01:45Cardiac Progenitor Cell (CPC) Generation
  • 03:13Heart Field-specific CPC Isolation by Fluorescent Protein Reporter Expression
  • 04:22Heart Field-specific CPC Isolation by Cell Surface Protein Marker Expression
  • 05:26Heart Field-Specific CPC Analysis
  • 07:18Results: Representative CPC Differentiation Characterization
  • 08:57Conclusion

Summary

Automatic Translation

Automatic Translation

The purpose of this method is to generate heart field-specific cardiac progenitor cells in vitro in order to study the progenitor cell specification and functional properties, and to generate chamber specific cardiac cells for heart disease modelling.

Tags

Heart Field-specific Cardiac Progenitor CellsMouse Embryonic Stem CellsCongenital Heart DiseaseCardiogenesisCardiac SpheroidsActivin ABone Morphogenetic Protein 4SFD Medium2i MediumIn Vitro Generation

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