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1Department of Molecular Medicine,Sapienza University, 2Department of Internal Medicine – DMISM,Sapienza University, 3Center for Life Nano Science@Sapienza,Istituto Italiano di Tecnologia, 4Pasteur Institute Italy-Fondazione Cenci Bolognetti, 5INSERM U1052-Cancer Research Center of Lyon, 6Department of Hepatology,Croix Rousse Hospital, Hospices Civils de Lyon
Chapters
- 00:04Title
- 00:32Thawing, Amplification, and Cryopreservation of HepaRG Cells
- 02:20Differentiation of HepaRG cells (Day 0 21) and Induction of Vesicular Steatosis (Day 21 26)
- 04:25Evaluation of Lipid Overloading and Steatosis Induction: Oil Red O Staining
- 06:04Evaluation of Lipid Overloading and Steatosis Induction: Bodipy Staining
- 07:02Results: Induction and Quantification of Vesicular Steatosis in HepaRG Cells
- 08:34Conclusion
I denne studien, beskriver vi en detaljert protokoll for inducing leveren flytande steatosis i differensiert HepaRG celler med fettsyrer salt natrium oleat og ansette metoder for påvisning og kvantifisering av lipid akkumulering, inkludert sammenhengende anti-Stokes Raman spredning (CARS) mikroskopi, cytofluorimetric analyse, olje rød O farging, og qPCR.
