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Detection of Retrotransposition Activity of Hot LINE-1s by Long-Distance Inverse PCR
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Chapters
- 00:00Title
- 01:41Designing Inverse Primers and Selecting Restriction Enzymes (Bioinformatics)
- 05:56Making Circular DNA Templates for Long-distance Inverse PCR: DNA Extraction and Quality Assessment
- 08:29Long-distance Inverse PCR: Determining Primer Annealing Temperature by Gradient PCR
- 10:25Representative Results of LDI-PCR
- 11:27Conclusion
This article outlines a simple PCR-based assay to monitor the activity of an active LINE-1 retrotransposon and to map de novo retrotranspositions in a given genome. Using the MCF7 cell line, we demonstrate herein how this method can be applied to detect activity of a LINE-1 located at 22q12.1.
