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Immunology and Infection

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活细胞荧光显微镜研究细菌的亚细胞蛋白定位和细胞形态变化

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Immunology and Infection

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JoVE Journal Immunology and Infection

Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria

Please note that all translations are automatically generated. Click here for the English version.

活细胞荧光显微镜研究细菌的亚细胞蛋白定位和细胞形态变化

DOI:

10.3791/59905-v

•

05:57 min

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November 23, 2019

•

Robert S. Brzozowski, Maria L. White, Prahathees J. Eswara

¹Department of Cell Biology, Microbiology and Molecular Biology,University of South Florida

Chapters

00:04Title
00:48Sample Preparation
01:39Imaging
03:34Deconvolution and Data Quantification
04:36Results: Representative Visualization and Quantification of Cell Division Inhibition
05:23Conclusion

Summary

Automatic Translation

本文提供了一个分步指南，以研究蛋白质亚细胞定位动力学，并使用高分辨率荧光显微镜监测葡萄球菌和金黄色葡萄球菌的形态变化。

Tags

Keywords: Live-cell Fluorescence Microscopy Subcellular Protein Localization Cell Morphology Changes Bacteria Sample Preparation Membrane Staining Dye Agarose Slab High-resolution Deconvolution Microscope 100x Oil Immersion Objective Resolve 3D Imaging Software Z-stacks Sample Thickness

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