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Live-Cell fluorescens mikroskopi å undersøke Subcellulære protein lokalisering og Cell morfologi endringer i bakterier
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria
DOI:

05:57 min

November 23, 2019

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Chapters

  • 00:04Title
  • 00:48Sample Preparation
  • 01:39Imaging
  • 03:34Deconvolution and Data Quantification
  • 04:36Results: Representative Visualization and Quantification of Cell Division Inhibition
  • 05:23Conclusion

Summary

Automatic Translation

Denne artikkelen gir en steg-for-steg guide til å undersøke protein subcellulære lokalisering dynamikk og overvåke morfologiske endringer ved hjelp av høyoppløselig fluorescens mikroskopi i Bacillus subtilis og Staphylococcus aureus.

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