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A Murine Cell Line Based Model of Chronic CDK9 Inhibition to Study Widespread Non-Genetic Transcriptional Elongation Defects (TE definitely ) in Cancers A Murine Cell Line Based Model of Chronic CDK9 Inhibition to Study Widespread Non-Genetic Transcriptional Elongation Defects (TEdefinitely) in Cancers
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Chapters
- 00:04Title
- 01:28Confirmatory Assay of mRNA Processing Defects in the Generated Mouse TEdeff
- 05:39Exploratory Assay of the Response of Mouse TEdeff Model to Antigen Specific Cytotoxic T-cell Attack
- 08:14Results: Confirmatory and Exploratory Assessment of TEdeff Cell Model
- 09:34Conclusion
Le protocole détaille un modèle in vitro de carcinome murine de l'allongement défectueux non génétique de transcription. Ici, l'inhibition chronique de CDK9 est employée pour réprimer l'allongement productif de l'ARN Pol II le long des gènes pro-inflammatoires de réponse pour imiter et étudier le phénomène cliniquement surdes tecertainement, présent dans environ 20% de tous les types de cancer.
