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来自胚胎GFP-表达小鼠的Ex Vivo Oculo运动切片培养,用于对Oculo运动神经外生长进行时移成像

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Ex Vivo Oculomotor Slice Culture from Embryonic GFP-Expressing Mice for Time-Lapse Imaging of Oculomotor Nerve Outgrowth

Please note that all translations are automatically generated. Click here for the English version.

来自胚胎GFP-表达小鼠的Ex Vivo Oculo运动切片培养,用于对Oculo运动神经外生长进行时移成像

DOI:

10.3791/59911-v

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06:04 min

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July 16, 2019

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Mary C. Whitman^2,3, Jessica L. Bell³, Elaine H. Nguyen³, Elizabeth C. Engle^2,3,4,5,6

¹Department of Ophthalmology,Boston Children’s Hospital, ²Department of Ophthalmology,Harvard Medical School, ³F.M. Kirby Neurobiology Center,Boston Children’s Hospital, ⁴Department of Neurology,Boston Children’s Hospital, ⁵Department of Neurology,Harvard Medical School, ⁶Howard Hughes Medical Institute

Chapters

00:04Title
00:51Pregnancy Confirmation and E10.5 Embryo Harvest and Slice Preparation
04:15Results: Representative Ex Vivo Oculomotor Slice Culture Development and Pitfalls
05:30Conclusion

Summary

Automatic Translation

前体切片测定允许实时成像奥库运动神经外生。切片通过嵌入E10.5 Isl MN:GFP胚胎在生长中,在振动体上切片,并在一个顶的培养箱中生长。斧子引导通路的作用是通过向培养培养物添加抑制剂来评估的。

Tags

Keywords: Ex Vivo Oculomotor Slice Culture Embryonic GFP-expressing Time-lapse Imaging Oculomotor Nerve Outgrowth Axon Guidance Ocular Motor System Vibratome Agarose Slice Buffer

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