Journal
/
Biology
/
使用共同分泌的Luciferase代理物测量相对胰岛素分泌
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Measuring Relative Insulin Secretion using a Co-Secreted Luciferase Surrogate
Please note that all translations are automatically generated. Click here for the English version.

使用共同分泌的Luciferase代理物测量相对胰岛素分泌

DOI:
10.3791/59926-v

•

05:58 min

•

June 25, 2019

•

,
1Department of Pharmacology,University of Texas Southwestern Medical Center

Chapters

  • 00:04Title
  • 01:01Reagent Preparation and InsGluc MIN6 Cell Culture
  • 02:05Glucose-stimulated Gaussia Luciferase Secretion Assay
  • 03:48Secreted Gaussia Luciferase Assay
  • 04:31Results: Insulin Secretion from MIN6 Beta Cells
  • 05:22Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了如何使用胰岛素相关的高辛比酶作为β细胞胰岛素分泌的代理,在中等通量下执行快速低成本荧光素酶测定。大多数发光板读取器和多通道移液器都可以进行测定。

Tags

Keywords: Insulin SecretionInsulin ReporterLuciferaseBeta CellMIN6 CellsKrebs-Ringer Bicarbonate BufferKRBHCell CultureDrug DiscoveryDiabetesProtein Secretion

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59926?article_type=v

研究与基于荧光素酶的记者基因检测膜生源
check_url/59926?article_type=v

测量细菌细胞的抗弯刚度，使用光学陷阱
check_url/59926?article_type=v

注射胰岛素和血淋巴的提取来衡量成人胰岛素敏感性果蝇</em
check_url/59926?article_type=v

一个定量测定胰岛素表达细胞集落形成先觉
check_url/59926?article_type=v

监测细胞自主的生物钟节律基因表达，利用荧光素酶生物发光记者
check_url/59926?article_type=v

使用自制双辉光萤光素酶检测的高通量筛选功能
check_url/59926?article_type=v

对糖皮质激素信号A化学筛选程序与斑马鱼幼虫的荧光素酶报告系统
check_url/59926?article_type=v

转染RAW264.7细胞用荧光素酶报告基因
check_url/59926?article_type=v

监测内质网钙稳态使用<em> Gaussia</em>荧光素酶SERCaMP
check_url/59926?article_type=v

测量的刚度<em>离体</em>鼠标主动脉用原子力显微镜

Read Article