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Måling af relativ insulin sekretion ved hjælp af en co-udskilt luciferase surrogat
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JoVE Journal Biology
Measuring Relative Insulin Secretion using a Co-Secreted Luciferase Surrogate

Måling af relativ insulin sekretion ved hjælp af en co-udskilt luciferase surrogat

DOI:
10.3791/59926-v

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05:58 min

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June 25, 2019

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,
1Department of Pharmacology,University of Texas Southwestern Medical Center

Chapters

  • 00:04Title
  • 01:01Reagent Preparation and InsGluc MIN6 Cell Culture
  • 02:05Glucose-stimulated Gaussia Luciferase Secretion Assay
  • 03:48Secreted Gaussia Luciferase Assay
  • 04:31Results: Insulin Secretion from MIN6 Beta Cells
  • 05:22Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol beskriver, hvordan man udfører hurtige lave omkostninger luciferase analyser på medium-gennemløb ved hjælp af en insulin-linked gaussia luciferase som en proxy for insulinsekretion fra beta celler. Analysen kan udføres med de fleste luminescens plade læsere og multikanalspipetter.

Tags

Insulin SecretionInsulin ReporterLuciferaseBeta CellMIN6 CellsKrebs-Ringer Bicarbonate BufferKRBHCell CultureDrug DiscoveryDiabetesProtein Secretion

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