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一种基于强健聚合酶链反应的测定法,用于定量易碎 X 智力迟钝-1 基因中的细胞氨酸-鸟-鸟-鸟- 三核苷酸重复
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Genetics
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JoVE Journal
Genetics
A Robust Polymerase Chain Reaction-based Assay for Quantifying Cytosine-guanine-guanine Trinucleotide Repeats in Fragile X Mental Retardation-1 Gene
Please note that all translations are automatically generated.
Click here for the English version.
一种基于强健聚合酶链反应的测定法,用于定量易碎 X 智力迟钝-1 基因中的细胞氨酸-鸟-鸟-鸟- 三核苷酸重复
DOI:
10.3791/59963-v
•
08:22 min
•
September 16, 2019
•
Huilin Wang*
1
,
Xiaofan Zhu*
2,3
,
Baoheng Gui*
3,4
,
Wan Chee Cheung
,
Mengmeng Shi
3
,
Zhenjun Yang
3
,
Ka Yin Kwok
,
Ricky Lim
,
Sanna Pietilä
,
Yuanfang Zhu
7
,
Kwong Wai Choy
3
1
Central Laboratory, Bao’an Maternity and Child Health Hospital
,
Jinan University
,
2
Department of Obstetrics and Gynaecology
,
The Chinese University of Hong Kong
,
3
Shenzhen Research Institute
,
The Chinese University of Hong Kong
,
4
Department of Genetics and Metabolism
,
Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region
,
5
PerkinElmer Diagnostics
,
6
Department of Obstetrics and Gynecology, Bao’an Maternity and Child Health Hospital
,
Jinan University
,
7
Maternal-Fetal Medicine Institute, Bao’an Maternity and Child Health Hospital
,
Jinan University
Chapters
00:04
Title
00:44
PCR Amplification
02:20
Purification of the PCR Products
03:52
Fragment Sizing of PCR Products
05:32
-SB”
06:18
Results: Quantification of CGG Repeats of FMR1
07:29
Conclusion
Summary
Automatic Translation
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Automatic Translation
精确和强大的聚合酶链反应测定,用于量化易碎 X 智力迟钝-1 基因中细胞氨酸-瓜氨酸-鸟-鸟三核苷酸重复,有助于分子诊断和筛选易碎 X 综合征和易碎 X 相关周转时间较短,设备投资时间缩短。
Tags
PCR Assay
Fragile X Mental Retardation-1 Gene
CGG Trinucleotide Repeats
Molecular Diagnosis
Fragile X Syndrome
Fragile X-related Disorders
Cost-effective
Rapid Reporting
DNA Sample Preparation
PCR Master Mix
PCR Cleanup
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