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一种基于强健聚合酶链反应的测定法,用于定量易碎 X 智力迟钝-1 基因中的细胞氨酸-鸟-鸟-鸟- 三核苷酸重复
JoVE Journal
Genetics
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JoVE Journal Genetics
A Robust Polymerase Chain Reaction-based Assay for Quantifying Cytosine-guanine-guanine Trinucleotide Repeats in Fragile X Mental Retardation-1 Gene
DOI:

08:22 min

September 16, 2019

, , , , , , , , , ,

Chapters

  • 00:04Title
  • 00:44PCR Amplification
  • 02:20Purification of the PCR Products
  • 03:52Fragment Sizing of PCR Products
  • 05:32-SB”
  • 06:18Results: Quantification of CGG Repeats of FMR1
  • 07:29Conclusion

Summary

Automatic Translation

精确和强大的聚合酶链反应测定,用于量化易碎 X 智力迟钝-1 基因中细胞氨酸-瓜氨酸-鸟-鸟三核苷酸重复,有助于分子诊断和筛选易碎 X 综合征和易碎 X 相关周转时间较短,设备投资时间缩短。

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