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Journal / Immunology and Infection / Use of an Influenza Antigen Microarray to Measure …
JoVE Journal
Immunology and Infection

This content is Open Access.

JoVE Journal Immunology and Infection
Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes
 

Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes

Article DOI: 10.3791/59973-v • 08:52 min • July 26th, 2019
July 26th, 2019 •
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1Division of Infectious Diseases, Department of Medicine, University of California Irvine Health, 2Vaccine Research and Development Center, Department of Physiology, University of California Irvine

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Summary
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We present a protocol for using a protein microarray constructed by printing influenza antigens onto nitrocellulose-coated slides to simultaneously probe serum for multiple antibody isotypes against over 250 antigens from different virus strains, thus allowing measurement of the breadth of serum antibodies across virus subtypes.

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Keywords: Influenza Antigen Microarray Antibody Breadth Virus Subtypes High-throughput Measurement Antibody Characterization Microarray Printing Antigen Printing Microarray Probing
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