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Journal
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Immunology and Infection
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使用流感抗原微阵列测量病毒亚型血清抗体的广度
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes
Please note that all translations are automatically generated.
Click here for the English version.
使用流感抗原微阵列测量病毒亚型血清抗体的广度
DOI:
10.3791/59973-v
•
08:52 min
•
July 26, 2019
•
Saahir Khan
,
Aarti Jain
,
Omid Taghavian
,
Rie Nakajima
,
Algis Jasinskas
,
Medalyn Supnet
,
Jiin Felgner
,
Jenny Davies
,
Rafael Ramiro de Assis
,
Sharon Jan
,
Joshua Obiero
,
Erwin Strahsburger
,
Egest J. Pone
,
Li Liang
,
D. Huw Davies
,
Philip L. Felgner
1
Division of Infectious Diseases, Department of Medicine
,
University of California Irvine Health
,
2
Vaccine Research and Development Center, Department of Physiology
,
University of California Irvine
Chapters
00:04
Title
01:27
Antigen Microarray Printing
03:01
Probe Sera Microarray Incubation
04:25
Quantum-Dot-Conjugated Secondary Antibody Labeling
05:16
Antibody Binding Capture
06:03
Antibody Binding Quantification
07:00
Results: Representative Influenza Antigen Microarray Analysis
08:03
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们提出了一种使用通过将流感抗原打印到硝基纤维素涂层幻灯片上构建的蛋白质微阵列的协议,以同时针对来自不同病毒株的 250 多种抗原检测血清的多种抗体等型,从而允许测量病毒亚型血清抗体的广度。
Tags
Influenza Antigen Microarray
Antibody Breadth
Virus Subtypes
High-throughput Measurement
Antibody Characterization
Microarray Printing
Antigen Printing
Microarray Probing
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