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Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models
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Immunology and Infection
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JoVE Journal Immunology and Infection
Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models

Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models

DOI:
10.3791/59977-v

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08:14 min

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October 03, 2019

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, , , , , , , ,
1Hematovascular Biology Center, Robert M. Berne Cardiovascular Research Center,University of Virginia School of Medicine

Chapters

  • 00:04Title
  • 00:46Generation and Purification of Lentivirus Particles
  • 03:08Isolation and Transduction of Lineage-negative Cells from Mouse Bone Marrow
  • 05:23Transplantation of Transduced Cells into Lethally Irradiated Mice
  • 05:39Evaluating the Chimerism of Peripheral Blood
  • 06:19Results: Lentiviral Transduction of Mouse Bone Marrow Lineage-negative Cells
  • 07:23Conclusion

Summary

Automatic Translation

Automatic Translation

Described are protocols for the highly efficient genome editing of murine hematopoietic stem and progenitor cells (HSPC) by the CRISPR/Cas9 system to rapidly develop mouse model systems with hematopoietic system-specific gene modifications.

Tags

LentiviralCRISPR/Cas9Genome EditingHematopoietic CellsDisease ModelsTransgene Delivery293T CellsViral TiterBone IsolationHematopoietic Stem Cells

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