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使用RNA测序识别循环RNA
JoVE Journal
Genetics
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JoVE Journal Genetics
Identification of Circular RNAs using RNA Sequencing
DOI:

08:25 min

November 14, 2019

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Chapters

  • 00:05Title
  • 01:01RNase R Treatment
  • 01:58RNA Purification
  • 03:41circRNA Library Preparation
  • 06:25Results: Circular RNA Detection
  • 07:39Conclusion

Summary

Automatic Translation

循环RNA(循环RNA)是非编码RNA,在转录调节和蛋白质之间的中介相互作用中可能发挥作用。在对构建环RNA测序库的不同参数进行评估后,利用RNase R预处理的搁浅总RNA库制备编制了一个协议,并在此提出。

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