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Enkelt celle Durotaxis analyse til vurdering mekanisk kontrol af cellulære bevægelse og relaterede signalering begivenheder
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JoVE Journal Biology
Single Cell Durotaxis Assay for Assessing Mechanical Control of Cellular Movement and Related Signaling Events

Enkelt celle Durotaxis analyse til vurdering mekanisk kontrol af cellulære bevægelse og relaterede signalering begivenheder

DOI:
10.3791/59995-v

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00:08 min

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August 27, 2019

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, , ,
1Department of Pharmacology,University of Vermont Larner College of Medicine, 2University of Vermont Cancer Center, 3Department of Molecular Physiology and Biophysics,University of Vermont Larner College of Medicine

Chapters

  • 00:04Title
  • 00:41Glass Coverslip Activation
  • 01:15Fluorescent Microbead Deposition and Hydrogel Casting
  • 02:38Hydrogel Activation and Extracellular Matrix Coating
  • 03:38Cell Plating and Micropipette Preparation
  • 05:51Micromanipulator Calibration and Durotaxis Assay
  • 07:01Results: Representative Durotactic Stimulation Responses and Protein Expression Analyses
  • 07:55Conclusion

Summary

Automatic Translation

Automatic Translation

Mekaniske kræfter er vigtige for at kontrollere celle migration. Denne protokol demonstrerer brugen af elastiske silicagelrogeler, der kan deformeres ved hjælp af en glas mikropipette og en micromanipulator for at stimulere celler med en lokal stivhed gradient til at fremkalde ændringer i cellestruktur og migration.

Tags

Keywords: Single CellDurotaxisMechanical MicroenvironmentCell SignalingCell MigrationHydrogelFluorescent Micro-beadsSulfo-SANPAHUV Activation

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