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1Division of Nephrology and Clinical Immunology,University Hospital RWTH Aachen, 2III. Department of Medicine,University Medical Center, Hamburg-Eppendorf, 3Department of Nephrology,Monash Health, 4Division of Nephrology and Hypertension,Oregon Health and Science University, 5Renal Section,Veterans Affairs Portland Health Care System, 6Fondation LeDucq Transatlantic Networks of Excellence, 7Department of Internal Medicine, Nephrology and Transplantation,Erasmus Medical Center
Chapters
- 00:04Title
- 01:11Fixation and Immunostaining of Mouse Kidneys
- 03:26Tissue Clearing and Imaging
- 04:13Results: Immunolabeled and Optically Cleared Kidney Tissue
- 05:23Conclusion
Kombinationen af antistof mærkning, optisk clearing, og avanceret lys mikroskopi tillader tredimensionel analyse af komplette strukturer eller organer. Beskrevet her er en simpel metode til at kombinere immunolabeling af tykke nyre skiver, optisk clearing med ethyl cinnamat, og confokale Imaging, der muliggør visualisering og kvantificering af tredimensionelle nyre strukturer.
