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Developmental Biology
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增强的绿色荧光蛋白测定,用于研究原神经元的神经质生长
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
An Enhanced Green Fluorescence Protein-based Assay for Studying Neurite Outgrowth in Primary Neurons
Please note that all translations are automatically generated.
Click here for the English version.
增强的绿色荧光蛋白测定,用于研究原神经元的神经质生长
DOI:
10.3791/60031-v
•
08:02 min
•
October 19, 2019
•
Wai Wa Ray Chan*
1
,
Wen Li*
1,2
,
Dik Long Dennis Chau*
1
,
Kwok-Fai Lau
1
School of Life Sciences
,
The Chinese University of Hong Kong, Hong Kong Special Administrative Region
,
2
Shenzhen Institutes of Advanced Technology
,
Chinese Academy of Sciences, Shenzhen
Chapters
00:04
Title
00:56
Coverslip Preparation
01:36
Rat Embryonic Neuron Dissection
02:51
Primary Cortical Neuron Culture
04:08
Cell Transfection and Fixation
05:16
Neurite Overgrowth Measurement
05:58
Results: Representative Neurite Growth Modulation
07:29
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在本报告中,我们描述了一个简单的方案,通过与EGFP和感兴趣的蛋白质共同转染,研究胚胎大鼠皮质神经元中的神经质外生。
Tags
EGFP
Neurite Outgrowth
Primary Neurons
Transfection
Neural Regeneration
Brain Trauma
Neurodegenerative Diseases
Poly-D-Lysine
Sprague Dawley Rat
Cerebral Hemispheres
Cortex
Trypsin EDTA
Maintenance Medium
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