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Radiosensitivity of Cancer Stem Cells in Lung Cancer Cell Lines
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Cancer Research
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JoVE Journal Cancer Research
Radiosensitivity of Cancer Stem Cells in Lung Cancer Cell Lines

Radiosensitivity of Cancer Stem Cells in Lung Cancer Cell Lines

DOI:
10.3791/60046-v

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00:09 min

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August 21, 2019

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, , , ,
1Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Radiation Oncology,Peking University Cancer Hospital and Institute

Chapters

  • 00:04Title
  • 01:25Cell Labeling
  • 04:16Colony Formation Assay
  • 07:05Results: Radiosensitivity of Cancer Stem Cells
  • 08:31Conclusion

Summary

Automatic Translation

Automatic Translation

The presence of cancer stem cells have been associated with relapse or poor outcomes after radiotherapy. This manuscript describes the methods to study the radiosensitivity of cancer stem cells in lung cancer cell lines.

Tags

Keywords: Cancer Stem CellsRadiosensitivityLung CancerFlow CytometrySphere Formation AssayColony Formation AssayRadioresistanceDNA Damage RepairReactive Oxygen SpeciesCell Cycle Arrest

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