Journal
/
Biochemistry
/
通过低温BN-MS分析进行高分辨率复杂分析
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal Biochemistry
High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
Please note that all translations are automatically generated. Click here for the English version.

通过低温BN-MS分析进行高分辨率复杂分析

DOI:
10.3791/60096-v

•

09:33 min

•

October 15, 2019

•

, , , , ,
1Institute of Physiology, Faculty of Medicine,University of Freiburg, Freiburg, Germany, 2Institute of Experimental and Clinical Pharmacology and Toxicology, Faculty of Medicine,University of Freiburg, Freiburg, Germany, 3Signalling Research Centres BIOSS and CIBSS, Freiburg, Germany, 4Logopharm GmbH, Germany

Chapters

  • 00:04Title
  • 01:16Preparative BN-PAGE
  • 03:44Gel Sampling and Digestion
  • 06:32Results: High-Resolution Complexome Profiling
  • 08:29Conclusion

Summary

Automatic Translation

Automatic Translation

提出了一种使用微体体进行多功能冷冻BN-MS协议，用于高分辨率复杂体分析。

Tags

Keywords: CsBN-MSProtein ComplexesMembrane ProteinsSignal TransductionNative Protein ComplexSubunit CompositionGradient GelPolymerizationLoading SlotsRunning BuffersProtein Solubilization

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/60096?article_type=v

使用SPHIRE的电子冷冻显微镜图像的高分辨率单粒子分析
check_url/60096?article_type=v

解决由蓝变性PAGE和蛋白质相关剖析亲和纯化的蛋白复合物
check_url/60096?article_type=v

用等压标记、广泛的液相色谱法、质谱法和软件辅助定量技术进行深层蛋白质组分析
check_url/60096?article_type=v

核糖体剖面对芽殖酵母中翻译的全基因组定量
check_url/60096?article_type=v

血管紧张蛋白II图解的硝酸分析与鉴定
check_url/60096?article_type=v

DNA序列识别利用高通量原酶分析的DNAPrimase
check_url/60096?article_type=v

通过基因表达分析的改良方法从谷物种子获取高质量的转录数据
check_url/60096?article_type=v

使用200 KV透射电子显微镜常规收集高分辨率冷冻电镜数据集
check_url/60096?article_type=v

高分辨率中子光谱研究蛋白质和水合水的皮秒纳秒动力学
check_url/60096?article_type=v

通过高分辨率呼吸测量法评估坐骨神经的线粒体功能

Read Article