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Journal / Neuroscience / Single Synapse Indicators of Glutamate Release…
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JoVE Journal Neuroscience
Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice
 

Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice

Article DOI: 10.3791/60113-v • 08:27 min • March 11th, 2020
March 11th, 2020 •
1, 1
1Synaptic Dysfunction Group, Neuroscience Research Center, Charité - University Medicine

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Summary
Automatic Translation

We present a protocol to evaluate the balance between glutamate release and clearance at single corticostriatal glutamatergic synapses in acute slices from adult mice. This protocol uses the fluorescent sensor iGluu for glutamate detection, a sCMOS camera for signal acquisition and a device for focal laser illumination.

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Keywords: Single Synapse Glutamate Release Glutamate Uptake Acute Brain Slices Huntington Mice One-photon Microscope Autofluorescence Correction Dorsal Striatum Stimulation Pipette Action Potential Dependent Release Ion Channel Blockers Membrane Receptors Tetrototoxin
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