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Journal / Developmental Biology / Microinjection d'ADN dans eyebuds in Xenopus…
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Developmental Biology

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JoVE Journal Developmental Biology
Microinjection d'ADN dans eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles
 
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Microinjection d'ADN dans eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles

Article DOI: 10.3791/60123-v • 06:32 min • September 4th, 2019
September 4th, 2019 •
1, 1, 1
1College of Osteopathic Medicine, Touro University California

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Ce protocole vise à démontrer comment microinjecter un mélange ADN/DOTAP dans les œilcs d'embryons de Xenopus laevis d'un jour, et comment imager et reconstruire la protéine fluorescente verte individuelle (GFP) exprimant des arborescences axonales optiques dans les midbrains tectal de intacts, les têtards Xenopus vivants.

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Biologie du développement Numéro 151 Xenopus laevis lipofection arborescences axonales optiques GFP imagerie microinjection oculaires
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