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Measuring Diurnal Rhythms in Autophagic and Proteasomal Flux

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Biology

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JoVE Journal Biology

Measuring Diurnal Rhythms in Autophagic and Proteasomal Flux

Measuring Diurnal Rhythms in Autophagic and Proteasomal Flux

DOI:

10.3791/60133-v

•

09:18 min

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September 17, 2019

•

Mikhail Ryzhikov, Anna Eubanks, Jeffrey A. Haspel

¹Division of Pulmonary and Critical Care Medicine,Washington University School of Medicine

Chapters

00:04Title
00:40Protease Inhibitor Administration
01:31Tissue Acquisition and Biochemical Fractionation
05:10Western Blotting
06:48Data Analysis
08:06Results: Representative Experimental Flux Results
08:36Conclusion

Summary

Automatic Translation

We describe our protocol for measuring biological rhythms in protein catabolism via autophagy and the proteasome in mouse liver.

Tags

Keywords: Autophagy Proteasomal Flux Circadian Rhythms Protein Catabolism Leupeptin Bortezomib Homogenization Buffer Dounce Homogenizer Post-nuclear Supernatant Protein Concentration

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