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Immunology and Infection
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Measurement of Chitinase Activity in Biological Samples
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Immunology and Infection
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JoVE Journal Immunology and Infection
Measurement of Chitinase Activity in Biological Samples

Measurement of Chitinase Activity in Biological Samples

DOI:
10.3791/60159-v

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00:03 min

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August 22, 2019

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, , , , ,
1Pulmonary, Critical Care and Sleep Medicine,Yale University School of Medicine

Chapters

  • 00:04Title
  • 00:51Chitinase Assay
  • 02:33Results: Chitinase Activity
  • 03:00Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a simple method to measure chitinase activity in biological fluids such as bronchoalveolar lavage or serum.

Tags

Chitinase ActivityBiological SamplesDiagnostic MarkerTherapeutic MarkerGaucher Disease4-methylumbelliferoneMcIlvain BufferChitin SubstrateFluorescenceStandard CurveBronchoalveolar LavageSerumTransgenic MiceCHIT-1 Gene

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