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An Ectopic Chemokine Expression Model for Testing Macrophage Recruitment In Vivo
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Chapters
- 00:04Title
- 00:52Fluorescent Whole Mount In Situ Hybridization (WISH) Combined with Immunostaining
- 05:33Live Imaging
- 08:25Results: Fluorescent WISH and Live Imaging
- 09:17Conclusion
To test the effect of a chemokine on macrophage recruitment in vivo, the whole mount in situ hybridization was used to detect the ectopic expression of the chemokine, and immunostaining was used to label macrophages. Live imaging was used for real-time observation of macrophage migration.
