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用于测试 Vivo 中巨噬菌体招募的异位化学表达模型
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Developmental Biology
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JoVE Journal Developmental Biology
An Ectopic Chemokine Expression Model for Testing Macrophage Recruitment In Vivo
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用于测试 Vivo 中巨噬菌体招募的异位化学表达模型

DOI:
10.3791/60161-v

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10:02 min

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September 25, 2019

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, ,
1Department of Developmental Biology, School of Basic Medical Sciences,Southern Medical University, 2Division of Cell, Developmental and Integrative Biology, School of Medicine,South China University of Technology

Chapters

  • 00:04Title
  • 00:52Fluorescent Whole Mount In Situ Hybridization (WISH) Combined with Immunostaining
  • 05:33Live Imaging
  • 08:25Results: Fluorescent WISH and Live Imaging
  • 09:17Conclusion

Summary

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为了测试化学金对体内巨噬细胞招募的影响，使用整个原位杂交检测化学体异位表达，免疫染色用于标记巨噬细胞。实时成像用于实时观察巨噬菌体迁移。

Tags

EctopicChemokineMacrophageRecruitmentIn VivoCell ChemotaxisZebrafishTGFABP-10AInterleukin-34TransgenicMpeg1:GFPFixationDehydrationRehydrationProteinase KHybridizationProbeFormamideSSCTBlocking BufferAnti-digoxigenin HRP Antibody

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