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Analyzing Satellite Cell Function During Skeletal Muscle Regeneration by Cardiotoxin Injury and Injection of Self-delivering siRNA In Vivo
JoVE Journal
Genetics
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JoVE Journal Genetics
Analyzing Satellite Cell Function During Skeletal Muscle Regeneration by Cardiotoxin Injury and Injection of Self-delivering siRNA In Vivo

Analyzing Satellite Cell Function During Skeletal Muscle Regeneration by Cardiotoxin Injury and Injection of Self-delivering siRNA In Vivo

DOI:
10.3791/60194-v

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06:37 min

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September 18, 2019

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, , , , ,
1Leibniz-Institute on Aging – Fritz-Lipmann-Institute

Chapters

  • 00:04Title
  • 00:42Cardiotoxin-induced Muscle Injury and Self-delivering Small Interfering (si)RNA Injection
  • 02:21Tibialis Anterior Muscle Harvest
  • 04:10Results: Representative Skeletal Muscle Injury and Regeneration Analyses
  • 06:02Conclusion

Summary

Automatic Translation

Automatic Translation

We describe an in vivo method to investigate the functional loss of a specific gene in satellite cells by using a combination of cardiotoxin mediated injury of skeletal muscle and the injection of a self-delivering siRNA.

Tags

Keywords: Satellite CellSkeletal Muscle RegenerationCardiotoxin InjurySelf-delivering SiRNAIn VivoGene FunctionMuscle Injury

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