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Nanoskopisk billeddannelse af humane vævs sektioner via fysisk og isotropisk ekspansion
JoVE Journal
Medicine
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JoVE Journal Medicine
Nanoscopic Imaging of Human Tissue Sections via Physical and Isotropic Expansion
DOI:

09:11 min

September 25, 2019

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Chapters

  • 00:05Title
  • 01:19Preparation of Archived and Freshly Prepared Clinical Tissue Slides for ExPath
  • 04:05In Situ Polymerization of Specimens
  • 05:16Sample Digestion and Imaging
  • 06:40Results: Multicolor Super-resolution Tissue Imaging using Expansion Pathology
  • 08:18Conclusion

Summary

Automatic Translation

Nanoskala-billeddannelse af kliniske vævsprøver kan forbedre forståelsen af sygdoms patogenesen. Ekspansion patologi (expath) er en version af ekspansion mikroskopi (exm), modificeret for kompatibilitet med standard kliniske vævsprøver, at udforske nanoskala konfiguration af biomolekyler ved hjælp af konventionelle diffraktion begrænset mikroskoper.

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