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Obtention of Giant Unilamellar Hybrid Vesicles by Electroformation and Measurement of their Mechanical Properties by Micropipette Aspiration
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JoVE Journal Chemistry
Obtention of Giant Unilamellar Hybrid Vesicles by Electroformation and Measurement of their Mechanical Properties by Micropipette Aspiration

Obtention of Giant Unilamellar Hybrid Vesicles by Electroformation and Measurement of their Mechanical Properties by Micropipette Aspiration

DOI:
10.3791/60199-v

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09:29 min

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January 19, 2020

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, ,
1Université de Bordeaux,CNRS, Bordeaux INP, LCPO, UMR 5629

Chapters

  • 00:04Title
  • 01:07Glass Micropipette Tip Coating
  • 01:55Electroformation Chamber Preparation
  • 03:06Giant Unilamellar Vesicle (GUV) Electroformation
  • 04:12Micromanipulation Setup
  • 06:30Micropipette Aspiration Experiment
  • 07:57Results: Representative Stress-Strain Data for Liposomes, Polymersome, and Hybrid Polymer/Lipid Vesicles
  • 08:44Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of the protocol is to reliably measure membrane mechanical properties of giant vesicles by micropipette aspiration.

Tags

Keywords: ElectroformationHybrid VesiclesMicropipette AspirationMembrane MechanicsPolymer Lipid VesiclesCapillary ActionITO SlidesPrestressGlucose-BSA SolutionOrganic Solvent

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