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Genetics
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神経膠芽腫における機能的関連miRNAの特徴付けと遺伝子治療のための人工クラスターへの工学
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Genetics
Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
Please note that all translations are automatically generated.
Click here for the English version.
神経膠芽腫における機能的関連miRNAの特徴付けと遺伝子治療のための人工クラスターへの工学
DOI:
10.3791/60215-v
•
09:40 min
•
October 04, 2019
•
Vivek Bhaskaran
,
Pierpaolo Peruzzi
1
Department of Neurosurgery
,
Brigham and Women’s Hospital-Harvard Medical School
Chapters
00:04
Title
01:19
Induction of Cell Differentiation
02:16
Validation of miRNA Clusters by Stress-specific Challenges
03:28
Characterization of The Functional Convergence of Clustered miRNAs
05:27
Assembly of miRNA Modules into a Polycistronic Transgenic Cluster
07:51
Results: Functional miRNA Module and Transgene Processing
08:40
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここで説明する、生物学的に相乗的なmiRNAとその組み立てモジュールを短いトランス遺伝子に特徴付けるプロトコルであり、遺伝子治療用途に対する同時過剰発現を可能にする。
Tags
MiRNA
MicroRNA
Glioblastoma
Gene Therapy
Artificial MiRNA Clusters
Neural Stem Cells
GBM-34 Glioblastoma Stem-like Cells
Temozolomide
Bioinformatics
Gene Expression
Cancer
Poly-D-lysine
Cell Culture
In Vitro
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