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Characterization of the Effects of Migrastatic Inhibitors on 3D Tumor Spheroid Invasion by High-resolution Confocal Microscopy
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Cancer Research
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JoVE Journal Cancer Research
Characterization of the Effects of Migrastatic Inhibitors on 3D Tumor Spheroid Invasion by High-resolution Confocal Microscopy

Characterization of the Effects of Migrastatic Inhibitors on 3D Tumor Spheroid Invasion by High-resolution Confocal Microscopy

DOI:
10.3791/60273-v

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09:17 min

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September 16, 2019

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, ,
1School of Applied Sciences,University of Huddersfield, 2Laboratory of Radiotherapy and Experimental Radiation Oncology, Humbertus Wald Tumorzentrum,University Cancer Center Hamburg, University Medical Center Hamburg-Eppendorf

Chapters

  • 00:04Title
  • 01:01Generation of Cell Spheroids
  • 02:15Collagen Invasion Assay
  • 04:15Preparation of Collagen Embedded Spheroids and Migratory Cells for Confocal Microscopy
  • 07:13Results: Cell Migration and Invasion
  • 08:20Conclusion

Summary

Automatic Translation

Automatic Translation

The effects of migrastatic inhibitors on glioma cancer cell migration in three-dimensional (3D) invasion assays using a histone deacetylase (HDAC) inhibitor are characterized by high-resolution confocal microscopy.

Tags

3D Tumor SpheroidMigrastatic InhibitorsConfocal MicroscopyCancer ResearchCytotoxic DrugsCell MigrationCell Proliferation3D Cell CultureGliomaCollagenCell Invasion

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