Journal
/
Neuroscience
/
Pålidelig isolering af Mikrofartøjer i central nervesystemet på tværs af fem hvirveldyr grupper
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Reliable Isolation of Central Nervous System Microvessels Across Five Vertebrate Groups

Pålidelig isolering af Mikrofartøjer i central nervesystemet på tværs af fem hvirveldyr grupper

DOI:
10.3791/60291-v

•

10:35 min

•

January 12, 2020

•

, , , ,
1Department of Anatomy, Physiology and Cell Biology,University of California Davis, 2University of Veterinary Medicine Hannover Foundation

Chapters

  • 00:04Title
  • 00:40Central Nervous System (CNS) Tissue Dissection
  • 02:35CNS Tissue Homogenization
  • 04:20Microvessel Purification
  • 05:57Microvessel Elution and Filtration
  • 07:44Results: Representative Microvessel Protein Expression
  • 09:57Conclusion

Summary

Automatic Translation

Automatic Translation

Målet med denne protokol er at isolere mikrofartøjer fra flere regioner i centralnervesystemet af lissencephalic og gyrencephalic hvirveldyr.

Tags

Microvessel IsolationCentral Nervous SystemVertebratesDissectionMeningesChoroid PlexusHomogenization

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Systemic and Local Drug Delivery for Treating Diseases of the Central Nervous System in Rodent Models

Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System

Multimodal Imaging af stamcelle implantation i det centrale nervesystem mus

Produktion af lentivirusvektorer til transduktion af celler fra det centrale nervesystem

Mærkning af enkeltceller i centralnervesystemet hos<em> Drosophila melanogaster</em

En skade Paradigm at undersøge centrale nervesystem reparation i<em> Drosophila</em

Direkte Intraventrikulær Delivery of Drugs til Rodent centrale nervesystem

In vivo Afhøring af centralnervesystemet Translatome af Polyribosome Fraktionering

In vivo optogenetic Stimulering af Rodent centralnervesystemet

Funktionel Evaluering af biologisk Neurotoksiner i Networked Kulturer af stamceller afledt centralnervesystemet neuroner

Read Article