Journal
/
Immunology and Infection
/
Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection

Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection

DOI:
10.3791/60297-v

•

08:34 min

•

January 08, 2020

•

, ,
1Progenesis Technologies, LLC, 2Department of Biomedical Sciences, Pediatrics,Joan C. Edwards School of Medicine at Marshall University

Chapters

  • 00:05Title
  • 01:24Detection of Double-crossover Recombinants of P. aeruginosa
  • 02:24Confirmation of Gene Deletion with Colony PCR
  • 03:07Visualization of Bioluminescence-labeled Bacteria in Mice
  • 05:42Inoculation of Animals with Bacterial Strains
  • 06:28Results: Evaluation of Attenuated P. aeruginosa Strain
  • 07:24Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe a simple and reproducible protocol of mouse model of infection to evaluate the attenuation of the genetically modified strains of Pseudomonas aeruginosa in comparison to the United States Food and Drug Administration (FDA)-approved Escherichia coli for commercial applications.

Tags

Keywords: Pseudomonas AeruginosaIn-frame Gene DeletionVirulence AttenuationMouse Infection ModelReproducibilityCrossover RecombinantColony PCRDouble Crossover RecombinantCarbenicillin SensitivitySucrose ResistanceGene Mutation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/60297?article_type=v

Pseudomonas aeruginosa and Saccharomyces cerevisiae Biofilm in Flow Cells
check_url/60297?article_type=v

Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung
check_url/60297?article_type=v

Long Term Chronic Pseudomonas aeruginosa Airway Infection in Mice
check_url/60297?article_type=v

Pseudomonas aeruginosa Induced Lung Injury Model
check_url/60297?article_type=v

Studying Microbial Communities In Vivo: A Model of Host-mediated Interaction Between Candida Albicans and Pseudomonas Aeruginosa in the Airways
check_url/60297?article_type=v

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors
check_url/60297?article_type=v

Come to the Light Side: In Vivo Monitoring of Pseudomonas aeruginosa Biofilm Infections in Chronic Wounds in a Diabetic Hairless Murine Model
check_url/60297?article_type=v

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
check_url/60297?article_type=v

Evaluating Virulence and Pathogenesis of Aeromonas Infection in a Caenorhabditis elegans Model
check_url/60297?article_type=v

A Delayed Inoculation Model of Chronic Pseudomonas aeruginosa Wound Infection

Read Article