Journal
/
Immunology and Infection
/
在伪单体中生成帧内基因删除突变体，并在简单小鼠感染模型中测试病毒衰减
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection
Please note that all translations are automatically generated. Click here for the English version.

在伪单体中生成帧内基因删除突变体，并在简单小鼠感染模型中测试病毒衰减

DOI:
10.3791/60297-v

•

08:34 min

•

January 08, 2020

•

, ,
1Progenesis Technologies, LLC, 2Department of Biomedical Sciences, Pediatrics,Joan C. Edwards School of Medicine at Marshall University

Chapters

  • 00:05Title
  • 01:24Detection of Double-crossover Recombinants of P. aeruginosa
  • 02:24Confirmation of Gene Deletion with Colony PCR
  • 03:07Visualization of Bioluminescence-labeled Bacteria in Mice
  • 05:42Inoculation of Animals with Bacterial Strains
  • 06:28Results: Evaluation of Attenuated P. aeruginosa Strain
  • 07:24Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们描述了一个简单和可重复的小鼠感染模型协议，以评估与美国食品和药物管理局（FDA）批准的大肠杆菌用于商业用途的假单抗病毒的遗传改性菌株的衰减。

Tags

Pseudomonas AeruginosaIn-frame Gene DeletionVirulence AttenuationMouse Infection ModelReproducibilityCrossover RecombinantColony PCRDouble Crossover RecombinantCarbenicillin SensitivitySucrose ResistanceGene Mutation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

铜绿假单胞菌和酿酒酵母流细胞生物膜

使用人工痰介质测试抗生素药效试验<em>绿脓杆菌</em>更多相关的肺囊肿性纤维化的条件

长期慢性<em>铜绿假单胞菌</em>在呼吸道感染小鼠

绿脓杆菌引起的肺损伤模型

研究微生物群落<em>在体内</em>：主机之间介互动的典范<em>白色念珠菌</em>和<em>铜绿假单胞菌</em>在航空

一种简单有效的方法构建突变牛痘病毒载体

来到光明的一面: 体内糖尿病无毛小鼠慢性伤口生物膜感染的监测

复制的有序, 非冗余图书馆的铜绿假单胞菌应变 PA14 座子插入突变体

线虫模型中气单胞菌感染的毒力及发病机制评价

慢性假单位病伤口感染的延迟接种模型

Read Article