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Generation af in-frame-Gensletnings mutanter i Pseudomonas aeruginosa og testning for virulens dæmpning i en simpel muse model af infektion
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Immunology and Infection
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JoVE Journal Immunology and Infection
Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection

Generation af in-frame-Gensletnings mutanter i Pseudomonas aeruginosa og testning for virulens dæmpning i en simpel muse model af infektion

DOI:
10.3791/60297-v

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08:34 min

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January 08, 2020

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, ,
1Progenesis Technologies, LLC, 2Department of Biomedical Sciences, Pediatrics,Joan C. Edwards School of Medicine at Marshall University

Chapters

  • 00:05Title
  • 01:24Detection of Double-crossover Recombinants of P. aeruginosa
  • 02:24Confirmation of Gene Deletion with Colony PCR
  • 03:07Visualization of Bioluminescence-labeled Bacteria in Mice
  • 05:42Inoculation of Animals with Bacterial Strains
  • 06:28Results: Evaluation of Attenuated P. aeruginosa Strain
  • 07:24Conclusion

Summary

Automatic Translation

Automatic Translation

Her beskriver vi en enkel og reproducerbar protokol af musemodel af infektion til at evaluere dæmpning af de genetisk modificerede stammer af Pseudomonas aeruginosa i forhold til USA Food and Drug Administration (FDA)-godkendt Escherichia coli til kommercielle applikationer.

Tags

Pseudomonas AeruginosaIn-frame Gene DeletionVirulence AttenuationMouse Infection ModelReproducibilityCrossover RecombinantColony PCRDouble Crossover RecombinantCarbenicillin SensitivitySucrose ResistanceGene Mutation

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