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Glomerular Outgrowth as an Ex Vivo Assay to Analyze Pathways Involved in Parietal Epithelial Cell Activation
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Glomerular Outgrowth as an Ex Vivo Assay to Analyze Pathways Involved in Parietal Epithelial Cell Activation

Glomerular Outgrowth as an Ex Vivo Assay to Analyze Pathways Involved in Parietal Epithelial Cell Activation

DOI:
10.3791/60324-v

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06:39 min

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August 19, 2020

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, , , , , ,
1Radboud Institute for Molecular Life Sciences, Department of Pathology,Radboud University Medical Center, 2Radboud Institute for Molecular Life Sciences, Amalia Children’s Hospital, Department of Paediatric Nephrology,Radboud University Medical Center, 3Radboud Institute for Health Sciences, Department of Nephrology,Radboud University Medical Center, 4Radboud Institute for Molecular Life Sciences, Department of Nephrology,Radboud University Medical Center

Chapters

  • 00:04Introduction
  • 00:39Mouse Kidney Dissection
  • 01:04Isolation of Glomeruli from Mouse Kidney
  • 02:25Culturing of Glomerular Outgrowth
  • 03:40Analysis of Parietal Epithelial Cell Proliferation
  • 04:47Results: Analysis of Glomerular Outgrowth
  • 06:08Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes a method for culturing and analyzing glomerular parietal epithelial cell outgrowths of encapsulated glomeruli isolated from mouse kidney. This method can be used to study pathways involved in parietal epithelial cell proliferation and migration.

Tags

Glomerular OutgrowthParietal Epithelial Cell ActivationKidneyEx Vivo AssayCellular ProcessesPrimary CellsGlomeruliKidney IsolationCell CultureMicroscopy

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