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Biology
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上頭頂皮細胞活性化に関与する経路を解析するエクスビボアッセイとしての糸球体成長
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JoVE Journal
Biology
Glomerular Outgrowth as an Ex Vivo Assay to Analyze Pathways Involved in Parietal Epithelial Cell Activation
Please note that all translations are automatically generated.
Click here for the English version.
上頭頂皮細胞活性化に関与する経路を解析するエクスビボアッセイとしての糸球体成長
DOI:
10.3791/60324-v
•
06:39 min
•
August 19, 2020
•
Jennifer Eymael
,
Laura Miesen
,
Fieke Mooren
,
Jitske Jansen
2
,
Jack Wetzels
,
Johan van der Vlag
,
Bart Smeets
1
Radboud Institute for Molecular Life Sciences, Department of Pathology
,
Radboud University Medical Center
,
2
Radboud Institute for Molecular Life Sciences, Amalia Children’s Hospital, Department of Paediatric Nephrology
,
Radboud University Medical Center
,
3
Radboud Institute for Health Sciences, Department of Nephrology
,
Radboud University Medical Center
,
4
Radboud Institute for Molecular Life Sciences, Department of Nephrology
,
Radboud University Medical Center
Chapters
00:04
Introduction
00:39
Mouse Kidney Dissection
01:04
Isolation of Glomeruli from Mouse Kidney
02:25
Culturing of Glomerular Outgrowth
03:40
Analysis of Parietal Epithelial Cell Proliferation
04:47
Results: Analysis of Glomerular Outgrowth
06:08
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本稿では、マウス腎臓から分離されたカプセル化糸球体上皮細胞の増殖を培養し、分析する方法について述べている。この方法は、頭頂皮細胞の増殖および移動に関与する経路を研究するために使用することができる。
Tags
Keywords: Glomerular Outgrowth
Parietal Epithelial Cell Activation
Kidney
Ex Vivo Assay
Cellular Processes
Primary Cells
Glomeruli
Kidney Isolation
Cell Culture
Microscopy
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