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Mønster for geometrien af humane embryonale stamcelle kolonier på kompatible substrater til kontrol af Vævsniveau mekanik
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1Graduate Program in Bioengineering,University of California San Francisco and University of California Berkeley, 2Center for Bioengineering and Tissue Regeneration, Department of Surgery,University of California San Francisco, 3Department of Mechanical Engineering,University of California Berkeley, 4Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research,University of California San Francisco, 5UCSF Comprehensive Cancer Center, Helen Diller Family Cancer Research Center,University of California San Francisco, 6Department of Anatomy, Department of Bioengineering and Therapeutic Sciences, and Department of Radiation Oncology,University of California San Francisco
Chapters
- 00:04Title
- 00:49Generation of Stencils for Patterning ECM Ligand
- 03:47Patterning ECM Ligand on Coverslips
- 04:46Transfer of Ligand to Polyacrylamide Gel
- 07:27Results: Geometry of Human Embryonic Stem Cell Colonies
- 09:04Conclusion
Ekstracellulære matrix ligander kan mønstrede på polyacrylamid silicagelrogeler for at muliggøre kulturen af humane embryonale stamceller i indelukkede kolonier på kompatible substrater. Denne metode kan kombineres med Traction Force mikroskopi og biokemiske analyser for at undersøge samspillet mellem vævs geometri, celle genererede kræfter og skæbne specifikation.
