Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Production, Crystallization, and Structure…
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biochemistry
Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO
 

Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO

Article DOI: 10.3791/60339-v • 13:02 min • December 28th, 2019
December 28th, 2019 •
1, 1, 1, 1
1Department of Chemistry, Dartmouth College

Chapters

Summary
Automatic Translation

We describe protocols for the structure determination of the IKK-binding domain of NEMO by X-ray crystallography. The methods include protein expression, purification and characterization as well as strategies for successful crystal optimization and structure determination of the protein in its unbound form.

Tags

Keywords: NEMO IKK-binding Domain Protein Production Crystallization Structure Determination Coiled-coil Adapters Protein Refolding Protein Concentration Aggregation Solubility French Press Cell Lysis Urea Protein Purification
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter