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JoVE Journal Genetics
Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues
 

Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues

Article DOI: 10.3791/60366-v • 11:54 min • October 20th, 2019
October 20th, 2019 •
1, 2, 2, 1,2,3
1Department of Experimental Medicine, McGill University, 2Department of Human Genetics, McGill University, 3Department of Obstetrics and Gynecology, McGill University

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Summary
Automatic Translation

Hydatidiform moles are abnormal human pregnancies with heterogeneous aetiologies that can be classified according to their morphological features and parental contribution to the molar genomes. Here, protocols of multiplex microsatellite DNA genotyping and flow cytometry of formalin-fixed paraffin-embedded molar tissues are described in detail, together with results’ interpretation and integration.

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Keywords: Microsatellite DNA Genotyping Flow Cytometry Ploidy Analysis Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues Contamination Maternal DNA Diagnosis H&E Staining Chorionic Villi DNA Extraction
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