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大鼠乳腺内皮细胞移植到间膜白脂肪垫
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Rat Mammary Epithelial Cell Transplantation into the Interscapular White Fat Pad
DOI:

10:41 min

March 04, 2020

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Chapters

  • 00:05Introduction
  • 00:44Harvesting Donor Rat Mammary Gland Epithelium
  • 01:35Extract Brain Tissue from Euthanized Donors
  • 03:04Digestion and Processing of Mammary Gland Extracts
  • 05:08Transplantation Procedure (Recipient Rat 4-5 Weeks of Age)
  • 06:53Assessment of Epithelial Outgrowth
  • 07:40Results: Cell Transplantation into the Interscapular White Fat Pad
  • 09:04Conclusion

Summary

Automatic Translation

本文介绍了一种移植方法,将供体大鼠乳腺上皮细胞移植到受体动物的细胞间白脂肪垫中。该方法可用于检查宿主和/或供体对乳腺上皮发育的影响,并消除预清除的需要,从而扩展了此技术的实用性。

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