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Automated Counterflow Centrifugal System for Small-Scale Cell Processing
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Bioengineering
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JoVE Journal Bioengineering
Automated Counterflow Centrifugal System for Small-Scale Cell Processing

Automated Counterflow Centrifugal System for Small-Scale Cell Processing

DOI:
10.3791/60423-v

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04:49 min

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December 12, 2019

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, , , , , , , ,
1The Ritchie Centre,Hudson Institute of Medical Research, 2Department of Obstetrics and Gynaecology,Monash University, 3Scinogy, 4Australian Regenerative Medicine Institute,Monash University

Chapters

  • 00:05Title
  • 00:34Cell Preparation
  • 01:16Automated Buffer Exchange Setup
  • 02:05Automated Buffer Exchange and Cell Collection and Sampling
  • 02:48Results: Representative Post-Processing Cell Viability and Function
  • 03:58Conclusion

Summary

Automatic Translation

Automatic Translation

Automation is key to upscaling and cost management in cell manufacturing. This manuscript describes the use of a counterflow centrifugal cell processing device for automating the buffer exchange and cell concentration steps for small-scale bioprocessing.

Tags

Keywords: Automated Buffer ExchangeCell ProcessingCentrifugal SystemSmall-scale ManufacturingCell ConcentrationCell RecoverySingle-use Processing KitGraphic User InterfaceProtocol ProgrammingTubing ClampsFluidized Cell Bed

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