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Bioengineering
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定量荧光显微镜为基础的单脂体测定,用于检测单个脂质体之间的成分不均匀性
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes
Please note that all translations are automatically generated.
Click here for the English version.
定量荧光显微镜为基础的单脂体测定,用于检测单个脂质体之间的成分不均匀性
DOI:
10.3791/60538-v
•
09:12 min
•
December 13, 2019
•
Rasmus Münter
3
,
Thomas Lars Andresen
2,3
,
Jannik Bruun Larsen
2,3
1
Center for Nanomedicine and Theranostics
,
Technical University of Denmark
,
2
Center for Intestinal Absorption and Transport of Biopharmaceuticals
,
Technical University of Denmark
,
3
Department of Health Technology
,
Technical University of Denmark
Chapters
00:04
Title
00:58
Liposome Preparation
02:14
Surface Preparation of Imaging Chamber
02:57
Liposome Immobilization
03:42
Image Acquisition and Data Analysis
05:21
Liposome Size Calibration
06:42
Results: Liposome Surface Immobilization and Compositional Inhomogeneity
08:05
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了脂体的制造,以及如何用荧光显微镜将这些脂体固定在表面上,并单独以大规模平行的方式成像。这允许定量种群单脂质体之间的大小和组成不均匀性。
Tags
Single Liposome
Liposome Composition
Liposome Inhomogeneity
Fluorescence Microscopy
Lipid Labeling
Lipid Mixing
Freeze-thaw
Extrusion
BSA Coating
Streptavidin
Single-molecule Detection
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