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角膜内皮细胞损失非侵入性激光辅助实验模型的发展
JoVE Journal
Medicine
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JoVE Journal Medicine
Development of a Noninvasive, Laser-Assisted Experimental Model of Corneal Endothelial Cell Loss
DOI:

06:23 min

April 24, 2020

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Chapters

  • 00:04Introduction
  • 00:44Corneal Tissue Laser Treatment and Culture
  • 02:46Histology Preparation
  • 03:45Hematoxylin and Eosin (H&E) Staining
  • 05:02Results: Representative Probability of Selective Corneal Endothelial Cell (CEC) Damage
  • 05:57Conclusion

Summary

Automatic Translation

在这里,我们提出了一个协议,从Descemet的膜(DM)分离角膜内皮细胞(CEC),使用一个镉:YAG(Nd:YAG)激光作为牛角病(BK)的外活体疾病模型。

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