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基因组编辑细胞系细胞融合，用于细胞结构和功能的扰动
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Developmental Biology
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JoVE Journal Developmental Biology
Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function
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基因组编辑细胞系细胞融合，用于细胞结构和功能的扰动

DOI:
10.3791/60550-v

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07:30 min

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December 07, 2019

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,
1Photonics Group, Department of Physics,Imperial College London, 2The Medical Research Council Cancer Unit, Hutchison/MRC Research Centre, Cambridge Biomedical Campus,University of Cambridge, 3Cambridge Institute for Medical Research, Cambridge Biomedical Campus,University of Cambridge

Chapters

  • 00:04Title
  • 00:29Differential Fluorescent Cell Labeling
  • 02:00Cell-Cell Fusion
  • 03:03Fused Cell Enrichment
  • 04:18Cell-Cell Fusion Immunofluorescent Staining and Imaging
  • 05:30Results: Representative Fused Cell Flow Cytometry Enrichment and Imaging
  • 06:47Conclusion

Summary

Automatic Translation

Automatic Translation

该协议的目的是融合两种不同的细胞类型，以创建混合细胞。熔融细胞的荧光显微镜分析用于跟踪细胞细胞器来源细胞。此测定可用于探索细胞结构和功能如何对细胞融合对扰动的反应。

Tags

Cell-cell FusionGenome EditingCellular StructureCellular FunctionFluorescent LabelingDye LabelingCell CultureCell FusionPolyethylene Glycol (PEG)Cell ImagingOrganelle HomeostasisOrganelle Function

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