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Monitoring PD-1-Blocking Antibodies Bound to T Cells Derived from a Drop of Peripheral Blood
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Monitoring PD-1-Blocking Antibodies Bound to T Cells Derived from a Drop of Peripheral Blood

Monitoring PD-1-Blocking Antibodies Bound to T Cells Derived from a Drop of Peripheral Blood

DOI:
10.3791/60608-v

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06:07 min

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February 05, 2020

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, , , , ,
1Department of Respiratory Medicine and Clinical Immunology,Osaka University Graduate School of Medicine

Chapters

  • 00:04Introduction
  • 00:35Whole Blood Sample Preparation and Staining
  • 02:22Flow Cytometric Analysis
  • 03:44Results: Flow Cytometry Analysis
  • 05:15Conclusion

Summary

Automatic Translation

Automatic Translation

We developed a simple flow cytometry assay for evaluating the binding of PD-1–blocking antibodies to T cells, requiring only a drop of peripheral blood from cancer patients.

Tags

Keywords: PD-1-blocking AntibodiesT CellsPeripheral BloodFlow CytometryCD8 T CellsCD4 T CellsPD-1Human IgG4Antitumor EffectsImmune-related Adverse EventsBlood SampleCell IsolationCell StainingData Analysis

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